Progress in Allergy Signal Research on Mast Cells:The Role of Histamine in Goblet Cell Hyperplasia in Allergic Airway Inflammation – a Study Using the Hdc Knockout Mouse

Journal of Pharmacological Sciences

Hiroshi Ohtsu⁴  Chihaya Maesawa³  Hitoshi Kobayashi²  Miyuki Niisato²  Yutaka Nakamura²  Kohei Yamauchi²  Toshihide Nakadate²  Hiroshi Inoue²  Takashi Mouri²  Toshiki Shikanai²  Hong Mei Piao²  Harumasa Ito²  Takashi Sawai¹ 

[1] First Department of Pathology, Iwate Medical University School of Medicine, Japan;Third Department of Internal Medicine, Iwate Medical University School of Medicine, Japan;Second Department of Pathology, Iwate Medical University School of Medicine, Japan;Tohoku University Graduate School of Engineering, Japan

关键词: airway remodeling;    asthma;    Gob-5;    MUC5AC;    allergy;   

DOI  :  10.1254/jphs.FM0070262

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(39)Cited-By(5)Although histamine is a central mediator in the immediate allergic reaction, its role in goblet cell hyperplasia in the airway of asthma is not completely understood. This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice (Hdc−/− mice) with allergic airway inflammation. Wild-type and Hdc−/− C57BL/6 mice were sensitized with ovalbumin (OVA). After two-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials in BALF were analyzed. The mRNAs level of MUC5AC and Gob-5 gene were quantitatively determined. The number of eosinophils in BALF increased in both the wild-type mice and Hdc−/− mice; however, their ratio in Hdc−/− mice was significantly lower than that in the wild-type mice. The mRNA levels of Gob-5 and MUC5AC and the ratio of the goblet cells in the airway epithelium were significantly increased in Hdc−/− mice exposed to OVA compared to the wild-type mice under the same condition. These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation.

【 授权许可】

Unknown