Immunohistochemistry, ultrastructure and pathology of gills of Abramis brama from Lake Mondsee,Austria, infected with Ergasilus sieboldi (Copepoda)

Diseases of Aquatic Organisms

Robert Konecny¹  Bahram Sayyaf Dezfuli¹  Luisa Giari¹  Paul Jaeger¹  Maurizio Manera¹ 

关键词: Ergasilus sieboldi infection;    Immunohistochemistry;    Ultrastructure;    Cellular responses;    Gill;    Abramis brama;   

DOI  :  10.3354/dao053257

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Immunohistochemical, ultrastructural and pathological studies were carried out on the gills of bream Abramis brama (L.) from Lake Mondsee, Austria, that were naturally infected with Ergasilus sieboldi Nordmann, 1832.Of a total of 14 specimens of bream examined, the gills of 7 (50%) were parasitized with this copepod and the intensity of infection ranged from 1 to 23 crustaceans per host. Histopathological investigations of infected gill showed extensive tissue damagedue to attachment and feeding of the crustacean. Parasites attached close to the base of filaments near the gill arch. Pressure exerted by the ectoparasite attached to the lateral margin of the gill filaments induced atrophy of the secondary lamellae.Tissue reactions included hyperplasia and mucous cell proliferation of the respiratory epithelium. Mucous cells displayed an intense immunohistochemical reactivity with the anti-nitric oxide synthase antibody. In parasitized primary and secondarylamellae, a high number of eosinophilic granular cells and rodlet cells were noticed. Rodlet cells represent an inflammatory cell type closely linked to other piscine inflammatory cells. Presence of a high number of inflammatory cells at the site of E.sieboldi attachment is related to intense host cellular reaction.

【 授权许可】

Unknown