Involvement of Sialic Acid in Transport of Serotype C1 Botulinum Toxins through Rat Intestinal Epithelial Cells

Journal of Veterinary Medical Science

Toshihiro WATANABE¹  Keita MIYATA¹  Hiroaki ITO¹  Ken INUI¹  Ryohta HORIUCHI¹  Tohru OHYAMA¹  Toshihiko IKEDA²  Tomohito MATSUO¹  Koichi NIWA¹ 

[1] Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture;Yokohama College of Pharmacy

关键词: botulinum neurotoxin;    Clostridium botulinum;    IEC-6;    toxin absorption;    toxin complex;   

DOI  :  10.1292/jvms.10-0090

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(8)Clostridium botulinum produces a large toxin complex (L-TC) composed of neurotoxin (BoNT) and non-toxic proteins. In animal botulism, BoNT or L-TC is absorbed via the intestinal epithelium. To establish the cellular mechanisms of botulinum toxin absorption, we used cultured rat intestinal epithelial cells to test the binding and transport of serotype C1 BoNT and L-TC through the cell layers. BoNT and L-TC bound to and passed through the cell layers, with L-TC exhibiting larger binding and transport. Binding and transport of these toxins were inhibited by N-acetyl neuraminic acid or neuraminidase treatment of the cells. These results suggest that binding of serotype C1 BoNT and L-TC to sialic acid on the cells promoted their transport through intestinal epithelial cell layers.

【 授权许可】

Unknown