Production of Ascaris suum As14 Protein and Its Fusion Protein with Cholera Toxin B Subunit in Rice Seeds

Journal of Veterinary Medical Science

Naotoshi TSUJI²  Takeshi ARAKAWA⁵  Yasunobu MATSUMOTO¹  Yoshihiro HAYASHI¹  Takashi YAMAKAWA⁴  Fumio TAKAIWA³  Tomoko NOZOYE¹ 

[1] Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization;Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences;Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Division of Molecular Microbiology, Center of Molecular Biosciences, University of the Ryukyus

关键词: Ascaris suum 14-kDa surface antigen (As14);    cholera toxin B subunit (CTB);    edible vaccine;    glutelin-B promoter;    rice seed;   

DOI  :  10.1292/jvms.71.995

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(9)Plants are attractive vaccine production and oral delivery systems. Cereals are excellent candidate for edible vaccines, which can express and store high levels of proteins for extended periods of time without degradation. In this study, we produced a 14-kDa protective surface antigen of Ascaris suum L3 larvae and its fusion chimera with a mucosal carrier molecule cholera toxin B subunit (CTB) in rice (Oryza sativa L.) under the control of the endosperm-specific glutelin-B promoter. We found that the recombinant protein expression levels reached 1.5 μg per seed, a comparably high amount as compared to previously reported transgenic rice expression experiments. Potentials of transgenic rice plants as a source of oral vaccines against swine roundworm are discussed.

【 授权许可】

Unknown