Inhibition of Mouse Hepatitis Virus Multiplication by an Oligonucleotide Complementary to the Leader RNA

Journal of Veterinary Medical Science

Akihiko MAEDA¹  Tadashi YAMASHITA¹  Masanobu HAYASHI¹  Shigeo NAMIOKA¹  Tetsuya MIZUTANI¹  Hiroshi ISOGAI¹ 

关键词: antisense oligonucleotide;    leader RNA;    MHV;    viral multiplication;   

DOI  :  10.1292/jvms.54.465

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(2)An oligonucleotide complementary to a leader RNA of positive-stranded mouse hepatitis virus (MHV) was tested for the effect on the viral multiplication in mouse DBT cells. A 14-mer antisense oligonucleotide contained a sequence complementary to the conserved pentanucleotide sequence, UCUAA, of the leader RNA. A treatment of MHV-infected cells with the antisense oligonucleotide at concentrations from 5 to 25 μM had an inhibitory effect on the viral multiplication and reduted the synthesis of viral specific mRNA and proteins. No inhibitory effect was observed when the cells were treated with sense oligonucleotide and oligonucleotide which contained unrelated sequences at concentrations from 1 to 10 μM. These results showed that antisense oligonucleotide against the leader RNA reduced the multiplication of positive-stranded RNA virus, MHV.

【 授权许可】

Unknown