Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV

Diseases of Aquatic Organisms

Ellen Lorenzen¹  , Niels Jørgen Olesen¹  Bendix Carstensen¹ 

关键词: VHSV;    IHNV;    IPNV;    CHSE-214;    FHM;    EPC;    BF-2;    RTG-2;    Cell line susceptibility;    Inter-laboratory comparison;   

DOI  :  10.3354/dao037081

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Eleven European National Reference Laboratories participated in an inter-laboratory comparison of the susceptibility of 5 selected cell lines to 3 fish pathogenic viruses. The test included viral hemorrhagic septicaemia virus (VHSV), infectioushematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), and the cell lines derived from bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papulosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad(RTG-2). The results showed that for isolation of VHSV, BF-2 and RTG-2 cells performed equally well and had higher sensitivity compared to the other cell lines. For IHNV, EPC and FHM cells gave the best results, and for IPNV it was BF-2 and CHSE-214cells. FHM cells showed the largest variability among laboratories, whereas EPC was the cell line showing the smallest variability.

【 授权许可】

Unknown