cDNA Cloning and mRNA Expression of Bovine GPR39

Journal of Veterinary Medical Science

Ichiro YAMAMOTO¹  Minoru TANAKA²  Nobuhiro KIMURA²  Toshiro ARAI¹ 

[1] Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Nippon Veterinary and Life Science University;Department of Animal Science, Faculty of Applied Life Science, Nippon Veterinary and Life Science University

关键词: bovine;    cDNA;    GPR39;    promoter;   

DOI  :  10.1292/jvms.71.641

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(2)GPR39 is an orphan G protein-coupled receptor that is thought to be involved in gastrointestinal and metabolic function. In this study, we cloned bovine GPR39 cDNA that encoded 462 amino acids showing high sequence homology to other mammalian GPR39 proteins. Real-time PCR showed expression of GPR39 mRNA in the liver, kidney, abomasums, small intestine, colon, rectum and uterus, with the highest level in the abomasums. Significant promoter activity was observed within the -2.3 kb 5'-upstream region of bovine GPR39 gene with human colon carcinoma-derived CACO-2 cells. These findings suggest that GPR39 may have important roles in gastrointestinal and metabolic functions in bovines as in other mammals.

【 授权许可】

Unknown