Characterization and evaluation of cholinesterase activity in the cockle Cerastoderma glaucum

Aquatic Biology

Sana Ben-Khedher¹  Hamadi Boussetta¹  Jamel Jebali¹  Nawel Kamel¹  Zied Bouraoui¹  Jihene Ghedira¹ 

关键词: Biomarker;    Cockle;    Cerastoderma glaucum;    Cholinesterase;    ChE;    Caging;    Fishing harbour;   

DOI  :  10.3354/ab00365

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The aim of the present study was to partially characterize the biochemical properties of cholinesterase (ChE) activity in the tissues of the cockle Cerastoderma glaucum, using different substrates and sample volumes, and to evaluate ChE response following caging exposure in a fishing harbour. Comparative analysis of the Michaelis-Menten kinetics in the studied tissues of C. glaucum indicated that ChE activity, using acetylthiocholine as substrate, displayed a tissue-dependent response, with activity levels in the following order of magnitude: whole animal > rest of animal (adductor and retractor muscles, mantle and foot) > visceral mass > gills. The transfer of C. glaucum to the Téboulba fishing harbour caused a time-dependent reduction in acetylcholinesterase activity.

【 授权许可】

Unknown