CpG motif in synthetic ODN primes respiratory burst of olive flounder Paralichthys olivaceus phagocytes and enhances protection against Edwardsiella tarda

Diseases of Aquatic Organisms

Hyung Ho Lee¹  Ki Hong Kim¹  Chan Hwei Lee¹  Hyun Do Jeong¹  Joon Ki Chung¹ 

关键词: Synthetic CpG ODN;    Olive flounder;    Respiratory burst;    Phagocytes;    Disease resistance;    Edwardsiella tarda;   

DOI  :  10.3354/dao056043

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Effects of synthetic cytidine-phosphate-guanosine (CpG) oligodeoxynucleotide (ODN) on respiratory burst activity of olive flounder (Paralichthys olivaceus) head-kidney phagocytes and on protection against lethal infection withEdwardsiella tarda were investigated. Phagocytes precultured with a CpG ODN showed significantly higher chemiluminescence (CL) responses than phagocytes precultured with guanosine-phosphate-cytidine (GpC) ODN or culture medium alone (control) atall concentrations. Supernatants produced from leucocytes, which were pulsed with CpG ODN, induced significantly higher respiratory burst activity than supernatants produced by GpC ODN or culture medium alone. In an in vivo experiment, respiratoryburst activities of the head kidney phagocytes in the groups injected either 0.25 or 0.5 µg fish^-1 of CpG ODN were significantly higher than those in the groups injected with GpC ODN or HBSS (control) at 3, 5 and 7 d after injection. The groupsof fish injected with 0.25 or 0.5 µg of CpG ODN showed higher survival rates (83.3%) than groups treated with GpC ODN (33.3%) and a control group (8.3%) after challenge with E. tarda. The present in vitro and in vivo experiments havedemonstrated the ability of synthetic CpG ODN to increase phagocyte respiratory burst activity and disease resistance in olive flounder.

【 授权许可】

Unknown