Interlaboratory variability in screening for Bonamia ostreae, a protistan parasite of the European flat oyster Ostrea edulis

Diseases of Aquatic Organisms

Matt Longshaw¹  Sarah C. Culloty¹  Andrea Ramilo¹  David Stone¹  Grace Flannery¹  Antonio Villalba¹  Paul Martin¹  Sharon A. Lynch¹ 

关键词: Bivalve;    Protozoa;    Diagnostics;    Methodology;    PCR;    In situ hybridisation;    Heart imprints;    Histology;   

DOI  :  10.3354/dao02717

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The spread of the protozoan parasite Bonamia ostreae is of major concern to the European flat oyster Ostrea edulis industry. Many studies have looked at the sensitivity of individual methods available to screen for B. ostreae, but in this study, 3 separate laboratories examined 4 methods of diagnosis currently used routinely in laboratories: heart imprints, histology, polymerase chain reaction (PCR) and in situ hybridisation (ISH). The results were compared to estimate interlaboratory variability. Heart imprints and histology had the highest reproducibility amongst the 3 laboratories, with greatest agreement between detection of infected and uninfected individuals. PCR had the highest detection level in every laboratory. These positives were related to the presence of confirmed infections but also in unconfirmed infections, possibly due to the presence of traces of B. ostreae DNA in oysters where clinical infections were not observed. PCR, in combination with histology or ISH, provided the most reliable detection levels in every laboratory. Variation in results for PCR and ISH observed between laboratories may be due to the different protocols used by each laboratory for both methods. Overall, the findings from the 3 laboratories indicated that at least 2 methods, with fixed protocols, should be used for the accurate detection and determination of infection prevalence within a sample. This combination of methods would allow for a clearer and more precise diagnosis of B. ostreae, preventing further spread of the disease and providing more accurate detection levels and epidemiological information.

【 授权许可】

Unknown