Molecular Epidemiology and Characterization of Genotypes of Acinetobacter baumannii Isolates from Regions of South China

Japanese Journal of Infectious Diseases

Li Zong¹  Zuyuan Xu¹  Liqiang Chen¹  Huiguang Yi¹  Qiyu Bao¹  Huifen Tou¹  Junwan Lu⁴  Teng Xu¹  Peizhen Li¹  Jun Ying¹  Kunpeng Li¹  Jinsong Li¹  Ailing Li¹  Liyan Ni²  Chuanxin Zhu³  Ruowang Pan⁵  Cong Cheng⁴  Jianchao Ying¹ 

[1] School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University;The Second Affiliated Hospital, Wenzhou Medical University;Wenzhou Center for Disease Control and Prevention;School of Medicine, Lishui College;118 Hospital of PLA

关键词: Acinetobacter baumannii;    drug resistance profiles;    molecular subtyping;    multilocus sequence typing;    pulsed-field gel electrophoresis;   

DOI  :  10.7883/yoken.JJID.2014.544

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(20)Cited-By(1)The aim of this study was to analyze the molecular epidemiologic characteristics of Acinetobacter baumannii. A total of 398 isolates were collected in 7 regions of South China from January to June of 2012. Drug sensitivity was tested toward 15 commonly used antibiotics; thus, 146 multi-drug-resistant strains (resistant to more than 7 drugs) were identified, representing 36.7% of all isolates. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular subtyping. According to the PFGE results (with a cutoff of 70% similarity for the DNA electrophoretic bands), 146 strains were subdivided into 15 clusters, with cluster A being the largest (33.6%, distributed in all districts except Jiaxing). Cluster B was also widespread and included 14.4% of all strains. In addition, MLST results revealed 11 sequence types (ST), with ST208 being the most prevalent, followed by ST191 and ST729. Furthermore, 4 novel alleles and 6 novel STs were identified. Our results showed that multi-drug-resistant A. baumannii in South China shares the origin with other widespread strains in other countries. The nosocomial infections caused by A. baumannii have been severe in South China. Continuous monitoring and judicious antibiotic use are required.

【 授权许可】

Unknown