In vivo analysis of compound activity and mechanism of action using epistasis in Drosophila

Journal of Chemical Biology

Marc Hild²  Erdem Bangi¹  Dan Garza³ 

[1] Developmental and Regenerative Biology, Mount Sinai Medical Center, New York, NY 10029 USA;Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research, Cambridge, MA 02139 USA;Proteostasis Therapeutics, Cambridge, MA 02139 USA

关键词: Chemical genetics;    Compound feeding;    Drosophila;    Drug discovery;    Signal transduction;   

DOI  :  10.1007/s12154-010-0051-5

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

The recent establishment of high-throughput methods for culturing Drosophila provided a unique ability to screen compound libraries against complex disease phenotypes in the context of whole animals. However, as compound studies in Drosophila have been limited so far, the degree of conservation of compound activity between Drosophila and vertebrates or the effectiveness of feeding as a compound delivery system is not well known. Our comprehensive in vivo analysis of 27 small molecules targeting seven signaling pathways in Drosophila revealed a high degree of conservation of compound activity between Drosophila and vertebrates. We also investigated the mechanism of action of AY9944, one of the Hh pathway antagonists that we identified in our compound feeding experiments. Our epistasis analysis of AY9944 provided novel insights into AY9944’s mechanism of action and revealed a novel role for cholesterol transport in Hh signal transduction.

【 授权许可】

Unknown