Development and maturation of the lung in fetuses ofGalea spixii and expression of markers

Ciência Rural

Favaron, Phelipe Oliveira²  Oliveira, Moacir Franco de¹  Anunciação, Adriana Raquel de Almeida²  Borghesi, Jéssica²  Miglino, Maria Angelica² 

[1] Universidade Federal Rural do Semi-Árido (UFERSA, Mossoró, Brazil;Universidade de São Paulo (USP, São Paulo, Brazil

关键词:  respiratory system;    alveoli;    morphology;    rodent;    experimental model;   

DOI  :  10.1590/0103-8478cr20150711

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:The aim of this research was to study the development of the lung inGalea spixii , by gross anatomy, histological analysis and immunohistochemical techniques. Totally, 8 fetuses were used and allocated into three groups of age: Group I (33-35 days), Group II (38-40 days), and Group III (43-45 days) with given crown-rump - CR lenghts. According to the gross morphology, there were no differences in relation to the lung morphology among groups. In relation to lung maturation, the Group I showed early formation of the bronchi and bronchioles, which were richly surrounded by mesenchyme and small blood vessels, typical features of the pseudoglandular stage. Individuals from Group II showed higher amounts of tubular formations in the lung parenchyma and reduced mesenchyme, reaching the canalicular stage. The lung from individuals of the Group III was completely formed, reaching the alveolar phase. In the immunohistochemical analysis, the lung of individuals from both Groups I and II were positive for Pcna, Oct-4, and VEGF. In contrast, there was not labbeling on samples from Group III. Thus, the G. spixiilung anatomy resembles the agouti ( Dasyproctasp.) lung, in relation to the number of lobes and fissures. In regard to the lung development, G. spixiishowed more similarity with the human fetal lung, since both are born in the stage of the alveolar phase, different to other rodent species.

【 授权许可】

Unknown