Asymbiotic seed germination and in vitro seedling development of Cattleya mendelii Dombrain (Orchidaceae)

Acta Agronómica

Salazar-Mercado, Seir Antonio¹ 

[1] Universidad Francisco de Paula Santander, San José de Cúcuta, Colombia

关键词: Cattleya mendelii;    culture medium;    embryonic development;    germination;    in vitro culture;    tetrazolium;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Colombia * Facultad de Ciencias Agropecuarias Palmira

【 摘 要 】

Cattleya mendelii is an endemic orchid species from Colombia, which has a great ornamental value which is in danger of extinction due to massive collection and their natural habitat's destruction by human activities. In vitro culture is an alternative to preserve this species and/or carry out its marketing. In this study the asymbiotic germination and seedling development of seeds of C. mendelii, in several in vitro culture media were evaluated. Mature capsules were collected. Seed viability with tetrazolium test was done; seeds were disinfected at the same time and planted by the syringe method to evaluate the effect of five growth culture media on the development of C. mendelii after 16 weeks of cultivation. It was found that seed viability was 93%, the highest percentage of germination was found in the culture medium Murashige-Skoog plus coconut water (MS+AC) with significant differences compared to other culture media (P < 0.05, Tu

【 授权许可】

Unknown