Indel-II region deletion sizes in the white spot syndrome virus genome correlate with shrimp disease outbreaks in southern Vietnam

Diseases of Aquatic Organisms

Just M. Vlak¹  Mark P. Zwart¹  Nguyen T. Phuong¹  Mart C. M. de Jong¹  Tran Thi Tuyet Hoa¹  Dang T. H. Oanh¹ 

关键词: White spot syndrome virus;    Disease status;    Farming system;    Epidemiology;    ORF 14/15;    ORF 23/24;   

DOI  :  10.3354/dao02463

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Sequence comparisons of the genomes of white spot syndrome virus (WSSV) strains have identified regions containing variable-length insertions/deletions (i.e. indels). Indel-I and Indel-II, positioned between open reading frames (ORFs) 14/15 and 23/24, respectively, are the largest and the most variable. Here we examined the nature of these 2 indel regions in 313 WSSV-infected Penaeus monodon shrimp collected between 2006 and 2009 from 76 aquaculture ponds in the Mekong Delta region of Vietnam. In the Indel-I region, 2 WSSV genotypes with deletions of either 5950 or 6031 bp in length compared with that of a reference strain from Thailand (WSSV-TH-96-II) were detected. In the Indel-II region, 4 WSSV genotypes with deletions of 8539, 10970, 11049 or 11866 bp in length compared with that of a reference strain from Taiwan (WSSV-TW) were detected, and the 8539 and 10970 bp genotypes predominated. Indel-II variants with longer deletions were found to correlate statistically with WSSV-diseased shrimp originating from more intensive farming systems. Like Indel-I lengths, Indel-II lengths also varied based on the Mekong Delta province from which farmed shrimp were collected.

【 授权许可】

Unknown