Changes in Extracellular Neurotransmitters in the Cerebrum of Familial Idiopathic Epileptic Shetland Sheepdogs Using an Intracerebral Microdialysis Technique and Immunohistochemical Study for Glutamate Metabolism

Journal of Veterinary Medical Science

Sigeo IKEDA²  Toshiyuki SHIBAHARA¹  Takashi TAKEUCHI³  Akinori SHIMADA²  Takehito MORITA²  Yoshiaki HIKASA⁴  Kota SATO⁴  Masumi SAWADA²  Makiko TAKAHASHI² 

[1] Laboratory Animal Research Center, Faculty of Medicine, Tottori University;Department of Veterinary Pathology, Tottori University;Department of Veterinary Physiology, Tottori University;Department of Veterinary Internal Medicine, Tottori University

关键词: canine;    epilepsy;    GLT-1;    glutamate;    neurotransmitter;   

DOI  :  10.1292/jvms.67.1119

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(7)Intracerebral microdialysis combined with electroencephalographic recordings was performed on 4 dogs of a familial idiopathic epileptic Shetland sheepdog colony to identify the kinds of neurotransmitters responsible for seizure activity. Immunohistochemistry using glutamate (Glu), glutamate transporter (GLT-1 and GLAST), and glutamine synthetase (GS) antibodies was also carried out on the cerebrum of four familial dogs that died of status epilepticus (SE). High values for extracellular levels of Glu and aspartate (ASP) were detected in association with an increased number of spikes and sharp waves during hyperventilation in 3 of 4 the familial epileptic dogs. The values of other amino acids analyzed were not altered in any of the familial epileptic dogs. Immunohistochemically, Glu-positive granules were occasionally found in the perineuronal spaces of the cerebral cortex in 3 of the familial epileptic dogs that died of SE. Immunostains for GLT-1 antibody predominantly decreased in the cerebral cortex and lateral nucleus of the thalamus in all the dogs that died of SE, whereas there were no differences detected in immunolabellings for GLAST and GS antibodies between familial epileptic dogs and controls. These results suggest that an extracellular release of both Glu and Asp may play an important role in the occurrence of seizure activity in this epileptic colony, and that a decreased expression of astrocytic GLT-1 may be related to development of SE.

【 授权许可】

Unknown