Molecular Analysis of Cryptosporidium parvum HNJ-1 Isolated in Japan

Journal of Veterinary Medical Science

Ryuma MATSUBARA¹  Yutaka NAKAI¹  Said AMER¹  Fumi MURAKOSHI¹ 

[1] Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University

关键词: Cryptosporidium parvum;    DHFR;    GP60;    HNJ-1;    ITS;   

DOI  :  10.1292/jvms.10-0118

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(1)Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.

【 授权许可】

Unknown