Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain | Science Publications

American Journal of Applied Sciences

Saeed Torkzadeh¹  Masoud A. Afzadi¹  Effat Behravan¹  Gholamreza Dehghan-Noudeh¹  Mohammad H. Moshafi¹ 

关键词: Pseudomonas aeruginosa;    biosurfactant;    surface tention;    emulsification index;   

DOI  :  10.3844/ajassp.2009.1453.1457

学科分类：自然科学（综合）

来源: Science Publications

【 摘 要 】

Problem statement: The chemical surfactants have some disadvantages; especially, toxicity and no biodegradability. Approach: Biosurfactants were the structurally diverse group of surface-active molecules synthesize by micro-organisms. The microbial surfactants were interesting, because of the biodegradable and have many applications in industry, agriculture, medicine. Results: In the present study, the production of biosurfactant by three strains of Pseudomonas aeruginosa (PTCC 1074, 1310 and 1430) was investigated. The hemolytic and foam forming activity of different strains were studied and consequently, P. aeruginosa PTCC 1074 was selected as the suitable strain. P. aeruginosa PTCC 1074 was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by emulsification index and surface tension for the best of production time. After that, in order to get maximum production of biosurfactant, the selected strain was grown with different additives in nutrient broth and the best culture medium was found. The biosurfactant was isolated from the supernatant and its amphipathic structure was confirmed by chemical methods. Conclusion: Biosurfactant produced by Pseudomonas aeruginosa PTCC 1074 would be considered as a suitable surfactant in industries due to its low toxicity.

【 授权许可】

Unknown