Detection of Vaccine-Like Infectious Bursal Disease (IBD) Virus in IBD Vaccine-Free Chickens in Japan

Journal of Veterinary Medical Science

Kenji OHYA³  Hideto FUKUSHI²  Tsuyoshi YAMAGUCHI¹  Min Thein MAW² 

[1] The Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University;Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University;Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University

关键词: chickens;    IBDV;    vaccine;   

DOI  :  10.1292/jvms.70.833

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(2)The prevalence of infectious bursal disease virus (IBDV) was studied in chickens, which had not been vaccinated against IBD. Fifty sera and forty-six bursae of Fabricius from chickens showing impaired growth, collected from 7 IBD vaccination-free farms in Japan were used for virus neutralization (VN) tests and RT-PCR for detection of IBDV genome corresponding to the VP2 hypervariable region. Of the fifty sera, 39 sera (78%) from 6 farms were VN antibodies positive. Of the forty-six bursae, 37 bursae (80.4%) from 6 farms were positive in the RT-PCR assay. The sequences of all the RT-PCR products detected in this study were closely related or identical to those of the vaccine strains. These results show that vaccine-like IBDV is prevalent even in IBD vaccine-free chicken farms in Japan.

【 授权许可】

Unknown