Diseases of Aquatic Organisms | |
Naked DNA vaccination of Atlantic salmon Salmo salar against IHNV | |
Bill Shewmaker1  Jon Richard1  Scott E. LaPatra1  Garth S. Traxler1  Gael Kurath1  Eric Anderson1  | |
关键词: DNA; Vaccine; G protein; Atlantic salmon; IHNV; | |
DOI : 10.3354/dao038183 | |
学科分类:生物科学(综合) | |
来源: Inter-Research | |
【 摘 要 】
ABSTRACT: A naked plasmid DNA encoding the glycoprotein (pCMV4-G) of a 1976 isolate of infectious hematopoietic necrosis virus (IHNV) obtained from steelhead Oncorhynchus mykiss was used to vaccinate Atlantic salmon Salmo salaragainst IHNV. Eight weeks post-vaccination the fish were challenged with a strain of IHNV originally isolated from farmed Atlantic salmon undergoing an epizootic. Fish injected with the glycoprotein-encoding plasmid were significantly (p < 0.05)protected against IHNV by both immersion and cohabitation challenge. Survivors of the first challenges were pooled and re-challenged by immersion 12 wk after the initial challenge. Significant (p < 0.05) protection was observed in all of the previouslychallenged groups including those receiving the complete vaccine. Fish injected with the glycoprotein-encoding plasmid produced low levels of virus-neutralizing antibodies prior to the first challenge. Neutralizing antibodies increased in all groupsafter exposure to the IHNV. Passive transfer of pooled sera from pCMV4-G vaccinates and IHN survivors provided relative survivals of 40 to 100% compared to fish injected with sera collected from fish immunized with control vaccines or left unhandled. Inthis study, DNA vaccination effectively protected Atlantic salmon smolts against challenges with IHNV.
【 授权许可】
Unknown
【 预 览 】
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RO201911300267529ZK.pdf | 699KB | download |