Real-time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss

Diseases of Aquatic Organisms

T. Lunder¹  D. Colquhoun¹  S. Grove¹  L. J. Reitan¹ 

关键词: Moritella viscosa;    Winter-ulcer;    Aquaculture;    Salmonids;    Norway;    Real-time PCR;   

DOI  :  10.3354/dao01972

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: We report the development of a real-time PCR protocol for specific detection of Moritella viscosa. This bacterium is considered to be the main aetiological agent in development of winter-ulcer, a disease severely affecting salmonid aquaculture in Norway. From a newly elaborated draft version of the genome of M. viscosa, the tonB gene sequence was selected as a suitable basis for the development of the real-time PCR assay. The real-time PCR demonstrated the presence of M. viscosa DNA sequences in 88.1% of samples collected from 35 outbreaks of winter-ulcer in Norwegian fish farms. In contrast, standard culturing on blood agar identified M. viscosa in only 39.7% of fish. While the culturing method revealed a similar prevalence (26 to 27%) of M. viscosa in kidney and ulcer samples, substantially more ulcer (81.5%) than kidney (49.7%) samples were shown positive by real-time PCR.

【 授权许可】

Unknown