Heat stress-mediated gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus

Aquatic Biology

Hongsheng Yang¹  Muyan Chen¹  Huan Zhao¹  Shilin Liu¹  Heling Zhao¹ 

关键词: Apostichopus japonicus;    Body wall;    Heat stress;    Suppression subtractive hybridization;    Stress response;   

DOI  :  10.3354/ab00315

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Suppression subtractive hybridization was used to analyze the differences in gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus, induced by heat stress (28°C), in order to understand the response at the molecular level. A total of 737 clones was selected and analyzed, and 165 distinct sequences were obtained, with 65 sharing similarity to sequences in public databases. The sequenced genes were classified into 3 functional categories. Increased expression of some genes in response to stress was concomitant with decreased expression of genes concerned with energy production and metabolism. The results (1) suggest that heat stress has significant effects on gene expression in A. japonicus, and (2) provide the basis for further study of molecular regulation mechanisms underlying the response to heat stress and estivation in A. japonicus.

【 授权许可】

Unknown