Development of a Multiplex PCR and PCR-RFLP Method for Serotyping of Avibacterium paragallinarum

Journal of Veterinary Medical Science

Masashi SAKAGUCHI¹  Ryuichi SAKAMOTO¹  Yoichiro KINO¹ 

[1]The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center

关键词: Avibacterium paragallinarum;    infectious coryza;    multiplex PCR;    RFLP;   

DOI  :  10.1292/jvms.11-0319

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(3)Avibacterium (Haemophilus) paragallinarum (A. paragallinarum) is a causative agent of infectious coryza in chickens and is classified into three serovars by agglutination tests. In an effort to identify the serovars easily, PCR and PCR-RFLP were employed. As the target gene for PCR, the hypervariable region of HMTp210, which encodes the HA antigen, was used. PCR using primer sets around the hypervariable region amplified 0.8, 1.1 and 1.6 kbp fragments for serovars A, B and C, respectively. Alternatively, the 1.6 kbp fragments were amplified with another primer pair encompassing the hypervariable region and was subjected to digestion with Bgl II, which resulted in the detection of serovar-specific digestion patterns. These results indicate that PCR and PCR-RFLP using the hypervariable region of HMTp210 are alternative methods to identify the serovar of A. paragallinarum.

【 授权许可】

Unknown