期刊论文详细信息
Journal of Veterinary Medical Science
Intraovarian Immunolocalization of Steroidogenic Enzymes in a Hokkaido Brown Bear, Ursus arctos yesoensis during the Mating Season
Toshio TSUBOTA1  Nobuhiro HARADA1  Isao KITA1  Hiroshi ARAKI1  Naoko MAEDA1  J. Ian. MASON1  Shiro KOMINAMI1 
关键词: bear;    immunocytochemistry;    ovary;   
DOI  :  10.1292/jvms.58.787
学科分类:兽医学
来源: Japanese Society of Veterinary Science
PDF
【 摘 要 】

References(12)Cited-By(5)Immunolocalization for four steroidogenic enzymes was performed on an ovary taken from a Hokkaido brown bear during the mating season. This specimen is considered to be in the follicular phase because of the presence of large follicles. In large follicles, cholesterol side-chain cleavage (P450scc) and 3β-hydroxysteroid dehydrogenase (3βHSD) were immunolocalized in theca interna cells and granulosa cells. 17α-hydroxylase/C17-C20 lyase cytochrome P450 (P450c17) was immunolocalized in theca interna cells but not in granulosa cells, Aromatase cytochrome P450 (P450arom) was immunolocalized only in granulosa cells. In medium follicles, however, P450scc and 3βHSD were immunolocalized only in theca interna cells, and the immunoreactivity of P450arom was detected in neither theca interna cells nor granulosa cells. Immunoreactivities of P450scc, 3βHSD and P450c17 but not P450arom were detected in interstitial cells. This study suggests that estrogen biosynthesis takes place through interrelation between theca cells and granulosa cells and is explained by the so-called two-cell mechanism. Furthermore, the granulosa cells in large follicles have the capability for pregnenolone and progesterone biosynthesis, and the interstitial cell in the bear ovary is also a steroidogenic site.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201911300257731ZK.pdf 1275KB PDF download
  文献评价指标  
  下载次数:8次 浏览次数:10次