Molecular characterisation of Neoparamoeba strains isolated from gills ofScophthalmus maximus

Diseases of Aquatic Organisms

Iva Dyková¹  Ivan Fiala¹ 

关键词: Paramoeba;    Neoparamoeba;    SSU rDNA;    Phylogenetic position;   

DOI  :  10.3354/dao055011

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Small subunit ribosomal RNA gene sequences were determined for 5 amoeba strains of the genus Neoparamoeba Page, 1987 that were isolated from gills of Scophthalmus maximus (Linnaeus, 1758). Phylogenetic analyses revealed that 2 of 5morphologically indistinguishable strains clustered with 6 strains identified previously as N. pemaquidensis (Page, 1970). Three strains branched as a clade separated from N. pemaquidenis and N. aestuarina (Page,1970) clades. Our analyses suggest that these 3 strains could be representatives of an independent species. In a more comprehensive eukaryotic tree, strains belonging to Neoparamoeba spp. formed a monophyletic group with a sister-grouprelationship to Vannella anglica Page, 1980. They did not cluster with Gymnamoebae of the families Hartmannellidae, Flabellulidae, Leptomyxidae or Amoebidae presently available in GenBank.

【 授权许可】

Unknown