Analysis of Urinary Nitric Oxide Metabolites in Healthy Subjects

Endocrine Journal

YOSHIO MURAKAMI¹  KUNIO KOSHIMURA¹  MIYOKO SUGITANI¹  SOICHI KURIOKA¹  MASATERU NISHIKI¹  YUZURU KATO¹ 

[1] First Division, Department of Medicine, Shimane Medical University

关键词: Nitric oxide (NO);    Nitrite (NO2-);    Nitrate (NO3-);    Urine;    Healthy subjects;   

DOI  :  10.1507/endocrj.46.421

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(30)Cited-By(16)Nitric oxide (NO) has divergent actions under physiological and pathological conditions. NO is rapidly decomposed to nitrite (NO2-) and nitrate (NO3-). Since these metabolites are stable, they are good indices of NO production under various conditions. In the present study, we measured NO2- and NO3- concentrations in the urine collected from 62 hospital controls and 504 healthy subjects by means of a new HPLC system combined with Griess reaction. NOx was the sum of NO2- and NO3- There was no considerable inter-day variation in urinary NO metabolite levels, and there was close correlation between NO2-, NO3- and NOx values in spot urine obtained in the early morning and those in 24-h stored urine in hospital controls. Urinary NO metabolite levels, which were corrected by creatinine (Cr) excretion and expressed on a logarithmic scale, showed normal distribution and were independent of sex and age in healthy subjects. The normal ranges of urinary NO2-, NO3- and NOx levels were estimated as 17-72μmol/g Cr, 1, 023-2, 818μmol/g Cr, and 1, 071-2, 951μmol/g Cr, respectively. We also found that urinary NO metabolite levels were lower than normal range in patients with various diseases.

【 授权许可】

Unknown