Indirect ELISA for the serodiagnostic of pythiosis

Pesquisa Veterinária Brasileira

Santurio, Janio M.¹  Leal, Alexandre Trindade²  Lübeck, Irina²  Leal, Adriana B. Monteiro²  Griebeler, Josiane²  Alves, Sydney Hartz¹  Copetti, Marina Venturini² 

[1] Universidade Federal de Santa Maria, Santa Maria;UFSM

关键词: Pythium insidiosum;    pythiosis;    ELISA;    horses;    rabbits.;   

DOI  :  10.1590/S0100-736X2006000100010

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Pythiosis is a granulomatous disease caused by the oomycete Pythium insidiosum that affects humans and animals, especially horses. Deaths are very often the consequence of incorrect or late diagnosis when animals no longer respond to treatment. This study aimed standardization of the ELISA assay for the serodiagnostic of pythiosis in horses and rabbits, in order to minimize errors and delays in the diagnosis of the disease. Sera of 72 healthy and 44 of by pythiosis affected horses were used for development and evaluation of the test. The ELISA for equine diagnostic showed 97.72% sensitivity, 90.27% specificity, 86% positive predictive value, 98.4% negative predictive value, and 93.1% efficiency. The rabbit test was standardized with 48 sera of healthy rabbits and 24 sera of rabbits immunized with P. insidiosum antigens. The results were 91.66 % sensitivity, 95.83% specificity, 91.66% positive predictive value, 95.83% negative predictive value, and 94.44% efficiency. It can be concluded that ELISA is a reliable test for diagnostic and serological monitoring of pythiosis. Index

【 授权许可】

CC BY-NC