期刊论文详细信息
Pesquisa Veterinária Brasileira
Indirect ELISA for the serodiagnostic of pythiosis
Santurio, Janio M.1  Leal, Alexandre Trindade2  Lübeck, Irina2  Leal, Adriana B. Monteiro2  Griebeler, Josiane2  Alves, Sydney Hartz1  Copetti, Marina Venturini2 
[1] Universidade Federal de Santa Maria, Santa Maria;UFSM
关键词: Pythium insidiosum;    pythiosis;    ELISA;    horses;    rabbits.;   
DOI  :  10.1590/S0100-736X2006000100010
来源: Colegio Brasileiro de Patologia Animal-CBPA
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【 摘 要 】

Pythiosis is a granulomatous disease caused by the oomycete Pythium insidiosum that affects humans and animals, especially horses. Deaths are very often the consequence of incorrect or late diagnosis when animals no longer respond to treatment. This study aimed standardization of the ELISA assay for the serodiagnostic of pythiosis in horses and rabbits, in order to minimize errors and delays in the diagnosis of the disease. Sera of 72 healthy and 44 of by pythiosis affected horses were used for development and evaluation of the test. The ELISA for equine diagnostic showed 97.72% sensitivity, 90.27% specificity, 86% positive predictive value, 98.4% negative predictive value, and 93.1% efficiency. The rabbit test was standardized with 48 sera of healthy rabbits and 24 sera of rabbits immunized with P. insidiosum antigens. The results were 91.66 % sensitivity, 95.83% specificity, 91.66% positive predictive value, 95.83% negative predictive value, and 94.44% efficiency. It can be concluded that ELISA is a reliable test for diagnostic and serological monitoring of pythiosis. Index

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CC BY-NC   

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