Distribution of Clostridium perfringensIsolates from Piglets in South Korea

Journal of Veterinary Medical Science

Yong-Kuk KWON²  Ki-Eun LEE²  Seong-Ho SHIN¹  Dong-Jun AN²  Ha-Young KIM²  Jae-Young SONG²  Seong-In LIM² 

[1]Optifarm Solution, Osong, Chungbuk 363�?951, South Korea

[2]Viral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430�?824, South Korea

关键词: Clostridium perfringens;    multiplex PCR;    piglet;    pulsed-field gel electrophoresis;    toxin;   

DOI  :  10.1292/jvms.13-0430

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Clostridium perfringens causes various digestive systemdisease symptoms in pigs. In the present study, 11 C. perfringensisolates were obtained from diarrheic piglets and 18 from healthy piglets. All of theC. perfringens isolates were shown to be type A using a multiplex PCRassay. The β2 toxin gene was detected in 27/29 C.perfringens isolates, i.e., 81% (9/11) of diarrheic piglets and 100% (18/18) ofhealthy piglets, and all of the genes had the same sequence. In conclusion, theβ2 toxin gene of C. perfringens was distributed widelyin Korean piglets regardless of the incidence of diarrhea, and there was no clearrelationship with enteric disease. A pulsed-field gel electrophoresis analysis of DNAdigested using SmaI demonstrated the non-clonal spread of C.perfringens isolates from piglets.

【 授权许可】

Unknown