Suppression of Histamine Signaling by Probiotic Lac-B: a Possible Mechanism of Its Anti-allergic Effect

Journal of Pharmacological Sciences

Hiroyuki Fukui²  Asish K. Das²  Jun Kojima¹  Takayuki Ohtoshi³  Hiroyuki Mizuguchi²  Shrabanti Dev²  Kunihiko Takahashi¹  Chiyo Matsushita²  Kazutaka Maeyama⁴  Hayato Umehara²  Kiyotaka Nishida¹ 

[1] Clinical Research Group, Clinical Development Department, Kowa Pharmaceutical Co., Ltd., Japan;Department of Molecular Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan;Ohtoshi Clinic, Japan;Division of Pharmacology, Department of Integrated Basic Medical Science, Ehime University School of Medicine, Japan

关键词: Lac-B;    histamine signaling;    histamine H1 receptor;    histidine decarboxylase;    probiotics;   

DOI  :  10.1254/jphs.08028FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(35)Cited-By(14)It has been shown that probiotic bacteria are effective for the treatment of allergic diseases. As histamine plays a central role in allergic diseases, it is possible that probiotic bacteria affect the allergy-related histamine signaling. Here, we investigated the effect of Lac-B, a mixture of freeze-dried Bifidobacterium infantis and Bifidobacterium longum, on the allergy-related histamine signaling. In the nasal allergy model rats made by sensitization and provocation with toluene 2,4-diisocyanate (TDI) for 3 weeks, TDI provocation caused acute allergy–like behaviors along with significant up-regulation of histamine H1 receptor (H1R) and histidine decarboxylase (HDC) mRNA expression, increased HDC activity, histamine content, and [3H]mepyramine binding activity in nasal mucosa. Prolonged treatment with Lac-B (40 mg/rat, p.o.) significantly suppressed both the allergy-like behaviors and all of the above mentioned factors involved in histamine signaling. Our findings indicate that oral administration of Lac-B showed significant anti-allergic effect through suppression of both H1R and HDC gene expression followed by decrease in H1R, HDC protein level, and histamine content. Suppression of histamine signaling may be a novel target of probiotics in preventing allergic diseases.

【 授权许可】

Unknown