Journal of Veterinary Medical Science | |
High antiviral effects of hibiscus tea extract on the H5 subtypes of low andhighly pathogenic avian influenza viruses | |
Haruko OGAWA1  Dai Q. TRINH1  Dulyatad GRONSANG1  Vuong N. BUI1  Emi YAMAGUCHI2  Kunitoshi IMAI1  Rapeewan THAMPAISARN1  Tugsbaatar BAATARTSOGT1  | |
[1] Diagnostic Center for Animal Health and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080�?8555, Japan;United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020�?8550, Japan | |
关键词: antiviral effect; H5N1; herbal tea extract; hibiscus; | |
DOI : 10.1292/jvms.16-0124 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(35)Viral neuraminidase inhibitors are widely used as synthetic anti-influenza drugs for theprevention and treatment of influenza. However, drug-resistant influenza A virus variants,including H5N1 highly pathogenic avian influenza viruses (HPAIVs), have been reported.Therefore, the discovery of novel and effective antiviral agents is warranted. We screenedthe antiviral effects of 11 herbal tea extracts (hibiscus, black tea, tencha, rosehip tea,burdock tea, green tea, jasmine tea, ginger tea, lavender tea, rose tea and oak tea)against the H5N1 HPAIV in vitro. Among the tested extracts, only thehibiscus extract and its fractionated extract (frHibis) highly and rapidly reduced thetiters of all H5 HPAIVs and low pathogenic AIVs (LPAIVs) used in the pre-treatment testsof Madin–Darby canine kidney (MDCK) cells that were inoculated with a mixture of the virusand the extract. Immunogold electron microscopy showed that anti-H5 monoclonal antibodiescould not bind to the deformed H5 virus particles pretreated with frHibis. Inpost-treatment tests of MDCK cells cultured in the presence of frHibis after infectionwith H5N1 HPAIV, the frHibis inhibited viral replication and the expression of viralantigens and genes. Among the plants tested, hibiscus showed the most prominent antiviraleffects against both H5 HPAIV and LPAIV.
【 授权许可】
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