Identification of a 27.8 kDa protein from flounder gill cells involved in lymphocystis disease virus binding and infection

Diseases of Aquatic Organisms

Jing Xing¹  Xiu-Zhen Sheng¹  Xiao-Qian Tang¹  Mu Wang¹  Wen-Bin Zhan¹ 

关键词: Lymphocystis disease virus;    Receptor;    Virus entry;    Flounder gill cells;    Co-immunoprecipitation;   

DOI  :  10.3354/dao02311

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: In vitro, lymphocystis disease virus (LCDV) infection of flounder gill (FG) cell cultures causes obvious cytopathic effect (CPE). We describe attempts to isolate and characterize the LCDV-binding molecule(s) on the plasma membrane of FG cells that were responsible for virus entry. The results showed that the co-immunoprecipitation assay detected a 27.8 kDa molecule from FG cells that bound to LCDV. In a blocking ELISA, pre-incubation of FG cell membrane proteins with the specific antiserum developed against the 27.8 kDa protein could block LCDV binding. Similarly, antiserum against 27.8 kDa protein could also inhibit LCDV infection of FG cells in vitro. Mass spectrometric analysis established that the 27.8 kDa protein and β-actin had a strong association. These results strongly supported the possibility that the 27.8 kDa protein was the putative receptor specific for LCDV infection of FG cells.

【 授权许可】

Unknown