Effect of lectins on the invasion of Ichthyophthirius theront to channel catfish tissue

Diseases of Aquatic Organisms

De-Hai Xu¹  Phillip H. Klesius¹  Craig A. Shoemaker¹ 

关键词: Lectins;    Ichthyophthirius;    Immobilization;    Invasion;    Development;    Tissue;   

DOI  :  10.3354/dao045115

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: This study determined the effects of lectin binding to theronts of Ichthyophthirius multifiliis on theront immobilization, invasion, trophont development and survival in channel catfish Ictalurus punctatus excised fins in vitro.Soybean agglutinin (SBA), lentil agglutinin (LCA), gorse agglutinin (UEA-I) and wheat germ agglutinin (WGA) were used to treat theronts. Percentages of theronts immobilized by 4 lectins ranged from 12.0 to 19.4% at a concentration of 1000 µgml^-1. These lectins bound more than half of the theronts at a concentration of 50 µg ml^-1. More theronts were labeled by SBA and WGA than by lectin LCA at concentrations of 50 and 100 µg ml^-1, respectively. The binding ofthese lectins to theronts indicated that monosaccharides (d-galactose, l-fucose, d mannose and d-glucose) and amino sugar derivatives (N-acetylgalactosamine and N-acetylglucosamine) were present on the surface of theronts. Invasion wasreduced significantly for theronts treated with LCA, UEA-I and WGA. No difference in invasion was found between control and SBA bound theronts (p > 0.05). The binding of lectin LCA, UEA-I and WGA to theronts significantly reduced the development oftrophonts (p < 0.05). The mean volumes of trophonts labeled with these 3 lectins were smaller than volumes in control trophonts from 8 to 48 h after exposure. Survival was lower in trophonts labeled with lectins than in control trophonts at 48 h afterexposure.

【 授权许可】

Unknown