Detection of Nesopora caninum-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

Journal of Veterinary Medical Science

Kazunori IKE³  Kyohei ISHIGAKI¹  Soichi IMAI³  Yumiko KAGAWA²  Hiromitsu ORIMA⁴  Masahiko NOYA¹ 

[1] Noya Animal Hospital, 143–19 Kamikayama, Hidaka, Saitama 350–1234, Japan;North Lab, 8–35 Kita, 2 Hondori, Shiroishi, Sapporo, Hokkaido 003–0027, Japan;Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan, Musashino, Tokyo 180–8602, Japan;Laboratory of Veterinary Radiology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan, Musashino, Tokyo 180–8602, Japan

关键词: antemortem diagnosis;    canine;    Neospora caninum;    neosporosis;    PCR;   

DOI  :  10.1292/jvms.11-0341

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(2)A one-month male Greyhound dog presented with a swinging gait of the hindlimbs, and later developed muscular atrophy of the femoral region and hyperextension of hindlimbs. The dog had positive serum IFAT titers to Neospora caninum, but a negative titer in the cerebrospinal fluid (CSF). N. caninum-specific DNA was amplified from the CSF using a semi-nested polymerase chain reaction assay. Clusters of protozoa in biopsied muscle fibers were subsequently confirmed as N. caninum tachyzoites by immunohistochemical examination. Early recognition and treatment are necessary for effective recovery of clinical canine neosporosis, but antemortem diagnosis is difficult. We suggest that the detection of parasite deoxyribonucleic acid in the CSF is a useful antemortem diagnostic method in facilitating treatment of this disease.

【 授权许可】

Unknown