Stablishment and in vitro multiplication of thornless blackberry (Rubus glaucus Benth.) by shoot apical meristems

Acta Agronómica

García-Delgado, Claudia Lucía¹  Sigarroa-Rieche, Alina Katil¹ 

[1] Universidad Francisco de Paula Santander, Cúcuta, Colombia

关键词: In vitro cultivation;    meristems;    micropropagation;    Rubus glaucus;    thornless blackberry;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Colombia * Facultad de Ciencias Agropecuarias Palmira

【 摘 要 】

We evaluated a technique of micropropagation in a thornless variety of blackberry (Rubus glaucus) from shoot apical meristems. In the establishment phase, we evaluated a disinfection protocol: soapy solution (commercial detergent and water) for 5 minutes + 70% alcohol for 2 minutes + 3% hypochlorite with two different exposure times: T1 for 5 minutes and T2 for 10 minutes. When the microcuttings were disinfected, the meristematic shoots were removed and established in vitro in a completely random design to evaluate two cultivation mediums: M1 and M2. From the seedlings developed, the multiplication was performed, which tested three cultivation mediums: M1, M3 and M4. Both disinfection treatments were effective achieving 100% disinfection of explants in each of them. Meristematic shoot planting allowed the establishment of aseptic cultivation and adequate development of the explants after six weeks of cultivation with rates of germination of 83.4% for M1 and 66.6% for M2. The analysis of variance (ANOVA) and multiple range test with Fisher's method (LSD), showed that multiplication the M1 medium favored better growth and development of the explant to obtain a multiplication coefficient of 7.5 shoots per seedling and an average height of 1.95 cm.

【 授权许可】

Unknown