Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe

Ciência Rural

Fajardo, Thor Vinícius Martins¹  Nickel, Osmar¹ 

[1] Embrapa Uva e Vinho, Bento Gonçalves, Brazil

关键词: diagnosis;    Malus;    Pyrus;    polyprobe;    ASGV;    ACLSV;    ASPV;    ApMV;   

DOI  :  10.1590/0103-8478cr20131629

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.

【 授权许可】

Unknown