Antimicrobial Resistance of Salmonella Serovars Isolated from Beef at Retail Markets in the North Vietnam

Journal of Veterinary Medical Science

Nguyen Thi LAN⁴  Takuya HIRAI¹  Truong Ha THAI¹  Pham Thi NGOC²  Akinori SHIMADA³  Ryoji YAMAGUCHI¹ 

[1] Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki,Miyazaki 889–2192, Japan;Department of Veterinary Hygiene, National of Institute Veterinary Research, Truong Chinh, Dong Da, Ha Noi, Vietnam;Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Tottori 680–8553, Japan;Department of Microbiology - Infectious Diseases - Pathology, Faculty of Veterinary Medicine, Hanoi University of Agriculture, Trau Quy, Gia Lam, Ha Noi, Vietnam

关键词: multidrug resistance;    North Vietnam;    quinolone resistance;    raw beef;    Salmonella;   

DOI  :  10.1292/jvms.12-0053

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(38)Cited-By(9)Approximately 39.9% (63/158) of beef samples collected from retail markets in Hanoi from January to June 2009 were Salmonella-positive. Nine Salmonella serovars, Anatum (28.6%), Rissen (25.4%), Weltevreden (12.7%), Typhimurium (7.9%), Derby (7.9%), Lexington (7.9%), Dublin (4.6%), Newport (3.2%) and London (1.8%), were identified. Thirty-seven (58.7%) of the 63 Salmonella isolates were resistant to at least one antimicrobial tested, of which 29 (46%) isolates showed multidrug resistance (MDR). The isolates were commonly resistant to tetracycline (46.0%), sulphonamide (39.7%), ampicilline (31.7%), streptomycin (30.2%), trimethoprim (28.6%), kanamycin (28.6%) and chloramphenicol (22.2%). Fourteen (blaTEMV, blaOXA-1, aadA1, aadA2, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac (3)-IV and aphA1-1AB) out of 22 antimicrobial resistance genes were detected by PCR from the resistant isolates. The catA1, Kn, blaPSE-1 genes and plasmid-mediated quinolones resistance (PMQR) genes such as qnrA, qnrB, qnrS, qepA and acc (6’)-ib-cr were not detected. Mutations in the gyrA gene leading to the amino acid changes Ser83Phe and/or Asp87Asn were found in 6 out of the 11 quinolone-resistant isolates. The data revealed that multidrug resistant Salmonella strains were widely distributed in north Vietnam via the food chain and might contain multiple genes specifying identical resistant phenotypes. Thus, continuous studies are necessary to clarify the mechanisms of MDR in Salmonella and its spread in the livestock market.

【 授权许可】

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