Detection of Haptoglobin in the High-Density Lipoprotein and the Very High-Density Lipoprotein Fractions from Sera of Calves with Experimental Pneumonia and Cows with Naturally Occurring Fatty Liver

Journal of Veterinary Medical Science

Hisami NAKAGAWA¹  Norio KATOH² 

[1] Ishikawa Nanbu Livestock Hygiene Service Center, Saida-machi, Kanazawa, Ishikawa 920-3101, Japan;Department of Systematic Diagnosis, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

关键词: bovine;    cholesteryl ester;    haptoglobin;    hemoglobin;    high-density lipoprotein;   

DOI  :  10.1292/jvms.61.119

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)Cited-By(20)In addition to the lipoprotein-deficient d >1.25 fraction, haptoglobin was detected in the high-density lipoprotein (HDL) and the very high-density lipoprotein (VHDL) fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver. It was not found in the chylomicrons, very low-density lipoprotein and low-density lipoprotein fractions. Washing of the HDL fraction did not decrease the haptoglobin concentration. Transferrin and immunoglobulin G were immunoblotted to examine the possibility of contamination of the lipoprotein fractions by the d >1.25 fraction. The two serum proteins were detected only in the d>1.25 fraction, not in any lipoprotein fractions. The distribution pattern of haptoglobin in the lipoprotein fractions was distinct from that of serum albumin. Concentrations of haptoglobin in the HDL fractions from pneumonic sera were largely proportional to those in whole sera. Cholesteryl ester concentrations were decreased in sera from calves with pneumonia, as in cows with fatty liver. A protein immunologically related to hemoglobin was also detected in particular in the VHDL fractions from sera of both groups. These results suggest that haptoglobin or a complex with the hemoglobin-like protein may have a role or roles related to the lipid metabolism.

【 授权许可】

Unknown