In vivo dose response and in vitro mechanistic analysis of enhanced immunoglobulin A production by Lactobacillus plantarum AYA

Bioscience of Microbiota, Food and Health

Kimiko OKITA¹  Shin-ichi FUKUDOME²  Tasuku OGITA³  Hikaru YOSHIDA³  Soichi TANABE³  Takuya SUZUKI³  Yosuke KIKUCHI² 

[1]Yeast Function Development Unit, Oriental Yeast Co., Ltd., Tokyo 174-8505, Japan

[2]Research Center for Basic Science, Research, and Development, Quality Assurance Division, Nisshin Seifun Group Inc., Saitama 356-8511, Japan

[3]Graduate School of Biosphere Science, Hiroshima University, Hiroshima 739-8528, Japan

关键词: Lactobacillus plantarum AYA;    Peyer’s patch;    Toll-like receptor 2;    immunoglobulin A;    cell wall;    probiotics;   

DOI  :  10.12938/bmfh.2014-016

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

References(25)Secretory immunoglobulin A (IgA) mediates the mucosal immune system, which provides the first line of defense against inhaled and ingested pathogenic bacteria and viruses. Lactobacillus plantarum AYA increases the IgA level of Peyer’s patch (PP) cells, but the recommended amount of consumption and the mechanism of action remains unclear. Better understanding of these is essential to development of L. plantarum AYA for use in functional foods. Therefore, we investigated the dose-response effect (in vivo) and mechanism (in vitro) of IgA enhancement induced by L. plantarum AYA. In the small intestine of the mice fed a diet containing 0.03% or 0.3% of L. plantarum AYA powder for 4 weeks, the IgA levels were significantly increased. Thus, it is suggested that the recommended amount of consumption of L. plantarum AYA is about 0.72 mg per day. In addition, the bacterial cell wall fraction significantly enhanced the IgA production level of murine PP cells in the in vitro assay. The ability of whole cells and the cell wall fraction to enhance IgA levels was significantly inhibited by an anti-Toll-like receptor-2 (TLR-2) antibody, which suggests that the cell wall fraction of L. plantarum AYA increases the IgA level via TLR-2. These findings indicate that L. plantarum AYA is a potential functional food source that maintains mucosal immunity.

【 授权许可】

Unknown