Diversity and molecular determination of wild yeasts in a central Washington State vineyard

North American Fungi

Dean A. Glawe¹  Thomas Henick-Kling¹  Gary G. Grove¹  Tyler B. Bourret¹  George J. Vandemark¹ 

关键词: fungi;    yeast;    grape;    Vitis;    phylogeny;    biodiversity;    systematics;    molecular determination;    ITS;    D1/D2;    rDNA;    Aureobasidium;    Candida;    Cryptococcus;    Curvibasidium;    Cystofilobasidium;    Hannaella;    Hanseniaspora;    Holtermanniella;    Metschnikowia;    Meyerozyma;    Mrak;   

DOI  :  

学科分类：植物学

来源: Pacific Northwest Fungi Project

【 摘 要 】

Yeasts were isolated from grapes collected from a research vineyard at the WSU-IAREC, located at Prosser, WA. Species determination was based on cultural features, microscopic morphology, physiological tests and analysis of ITS and D1/D2 rDNA sequence data. Fifty-three species were found distributed among five fungal subphyla, a greater number than expected based on similar published studies. Within Saccharomycotina, 13 species in the generaCandida, Hanseniaspora, Metschnikowia, Meyerozyma, Pichia, WickerhamomycesandYamadazymawere determined. Isolates within theMetschnikowia pulcherrimaclade appeared to possess considerable diversity. Pucciniomycotina was represented by 12 species, inCurvibasidium ,Rhodosporidium, Rhodotorula, SporidiobolusandSporobolomyces . Five phylogenetically distinct species in the subphylum could not be assigned to any described species. Isolates in Ustilaginomycotina were placed inPseudozymaexcept for a single strain determined to beRhodotorula bacarum .Within Agaricomycotina, 17 species in the generaCryptococcus, Cystofilobasidium ,Hannaella, HoltermanniellaandMrakiellawere determined. Seven species of yeast-like Pezizomycotina were found, representing classes Leotiomycetes, Dothideomycetes and Sordariomycetes. Isolates ofAureobasidium pullulansrepresented three phylogenetically distinct subspecific lineages.Seventeen species identified in this study were previously unreported from wine grapes and 18 species were unreported from North America. Several strains appear to represent undescribed species, including the recently describedCurvibasidium rogersii . 

【 授权许可】

Unknown