Transferibilidade de primers heterólogos e acesso ao polimorfismo de loci microssatélites em maracujazeiro-do-sono (Passiflora setacea DC)

Biotemas

Fernanda Amato Gaiotto²  Ronan Xavier Corrêa²  Antonio Carlos de Oliveira³  Douglas de Almeida Pereira¹ 

[1] Universidade Estadual do Sudoeste da Bahia (UESB);Universidade Estadual de Santa Cruz;Universidade Estadual do Sudoeste da Bahia

关键词: Diversidade genética;    Maracujazeiro-do-sono;    Simple sequence repeat;    Transferibilidade;   

DOI  :  

学科分类：生物科学（综合）

来源: Universidade Federal de Santa Catarina * Centro de Ciencias Biologicas

【 摘 要 】

http://dx.doi.org/10.5007/2175-7925.2015v28n3p51Primer pairs that access microsatellite loci, initially constructed through the genome ofPassiflora edulisSimsflavicarpaandP. alata , were tested concerning their ability to access microsatellite loci in ‘somnus�? passion fruit tree ( P. setacea ) individuals. Seven out of the thirty one primer pairs tested were able to access DNA polymorphism in the genome of this wildPassifloraspecies, by evaluating six natural populations, located in a transition area between the biomes Caatinga and Cerrado, in the state of Bahia, Brazil. The number of alleles/loci was small, oscillating from 1 to 4. The average heterozygosity observed per locus in all populations ranged from 0.13 to 0.40. There was transference of heterologous microsatellite primer pairs from thePassifloragenus to ‘somnus�? passion fruit tree, constituting a new set of primers that access random co-dominant locus in this species, useful for conservationist purposes and pre-improvement of ‘somnus�? passion fruit tree. 

【 授权许可】

Unknown