Infection prevalence and phylogenetic analysis of Perkinsus olseni in Ruditapes philippinarum from East China

Diseases of Aquatic Organisms

Jun-hua Deng¹  Shao-qiang Wu¹  Xiao-fei Li¹  Song-yin Qiu¹  Xiang-mei Lin¹  Jian Liu¹  Zong-xiang Wang¹  Cai-xia Wang¹ 

关键词: Perkinsus olseni;    Ruditapes philippinarum;    Infection prevalence;    Phylogenetic analysis;    China;   

DOI  :  10.3354/dao02353

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The prevalence of Perkinsus sp. infection in Manila clam Ruditapes philippinarum was investigated in the coastal areas of east China. Thirteen groups of clams were collected from 5 sites: Dandong and Qingdao Bays (Yellow Sea), Weifang Bay (Bohai Sea), and Ningbo and Fuzhou Bays (East China Sea). The clams were tested for perkinsosis infection using Ray’s fluid thioglycollate medium culture assay. Perkinsus sp. was found in samples from all 5 sites from May 2008 to May 2009. Infection prevalence ranged from 43.75 to 95.83%, and was significantly higher in October than in May. The only 3 uninfected groups of clams were collected from Weifang Bay, the site farthest from the ocean. There was no difference in the prevalence of infection among the remaining 4 sites. The conserved internal transcribed spacer regions of the ribosomal RNA gene complex in each of the Perkinsus sp. isolates were amplified by PCR. The resulting amplicons were sequenced and phylogenetically analyzed. All the Perkinsus isolates were identified as Perkinsus olseni.

【 授权许可】

Unknown