Evaluation of membrane filtration system using The “Pore Diffusion�? foreliminating viruses

Journal of Veterinary Medical Science

Akira HASHIMOTO-GOTOH¹  Takahiro MATSUKI²  Takayuki MIYAZAWA¹ 

[1] Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Kyoto 606�?8507, Japan;Opening Office for Biochemistry Business, Kasyu Industries Co., Ltd., 2�?5�?3 Minami-Futajima, Wakamatsu-ku, Kitakyusyu, Fukuoka 808�?0109, Japan

关键词: brownian motion;    laminar flow;    The Pore Diffusion;    trans-membrane pressure;    turbulence flow;   

DOI  :  10.1292/jvms.14-0625

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Here, we report a first study of virus removal by a novel membrane filtration system,named the “Pore Diffusion�?. The “Pore Diffusion�? manipulated the direction of circulatingflow from vertical to parallel to the membrane, thereby achieved to alter thetrans-membrane pressure as low as possible. We compared the viral activity between beforeand after filtration by both infectivity assay and real-time reverse transcription-PCR.Among 4 “Pore Diffusion�? modules tested, the big module with average pore size of 80 nmshowed the highest log reduction value of viral activity. Our study shows the possibilityof “The Pore Diffusion�? to filtrate viruses from bioproducts without increasing thetrans-membrane pressure, so that the filtration process can be carried out effectively andeconomically.

【 授权许可】

Unknown