Biological Activities of Lipopolysaccharides Extracted from Porcine Vaccine Strains

Journal of Veterinary Medical Science

Mari NORIMATSU¹  Yoshimasa SASAKI¹  Yutaka TAMURA¹  Yasuaki OGIKUBO¹  Akemi KOJIMA¹ 

[1] National Veterinary Assay Laboratory, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan

关键词: Actinobacillus pleuropneumoniae;    biological activity;    Bordetella bronchiseptica;    Haemophilus parasuis;    lipopolysaccharide;   

DOI  :  10.1292/jvms.61.1265

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(5)Lipopolysaccharides (LPSs) were purified from Actinobacillus pleuropneumoniae serotype 2, Bordetella bronchiseptica and Haemophilus parasuis serotype 5, which were used for vaccine production in Japan, by the phenol-water procedure. In SDS-PAGE analysis, A. pleuropneumoniae LPS, as well as Escherichia coli LPS, demonstrated a typical ladder profile of a smooth-type LPS. On the other hand, B. bronchiseptica and H. parasuis LPSs lacked the ladder profiles. It was found that the biological activity of these LPSs was comparable to those of E. coli LPS in terms of activation of the clotting enzyme of Limulus amoebocyte lysate, mitogenic activity of mouse spleen cells, stimulation of TNF-α and nitric oxide production, but IL-6 production could hardly be observed in any LPS.

【 授权许可】

Unknown