Evidence for the Expression of Alcohol Dehydrogenase Class I Gene in Rat Uterus and Its Up-regulation by Progesterone

Endocrine Journal

Osamu TSUTSUMI¹  Yumi HOSOKAWA¹  Fumiko NAKAZAWA¹  Tetsu YANO¹  Yuji TAKETANI¹  Minako KOIZUMI¹  Ryo TSUTSUMI¹  Tomoko OHNO¹  Mikio MOMOEDA¹  Hisahiko HIROI¹ 

[1] Department of Obstetrics and Gynecology, University of Tokyo

关键词: Progesterone;    Alcohol dehydrogenase;    Rat uterus;   

DOI  :  10.1507/endocrj.K07-082

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(50)Cited-By(4)The endometrium is one of the target tissues of the ovarian steroid hormones, estrogen and progesterone. In order to elucidate the mechanism of gene regulation in the endometrium, suppressive subtraction hybridization was performed to isolate the candidate genes controlled by progesterone in rat uterus. Alcohol dehydrogenase (ADH) class I gene was one of the candidate genes. Here we investigated the expression and regulation of ADH class I gene in rat uterus. The mRNA of ADH class I was detected in uterus by RT-PCR using specific primers. Using specific probe for ADH class I, in situ hybridization was performed to investigate localization in rat uterus. Positive signals were detected in the endometrial stromal cells of rat uterus by in situ hybridization and were not detected in endometrial epithelial cells and myometrium in rat uterus. Ovariectomized rats were treated with 17-β estradiol and progesterone and the uteri of these rats were used for Northern blot analysis and assay of the ADH activity. Northern blot analysis revealed that the expression of ADH class I mRNA in rat uteri was up-regulated approximately two-fold after progesterone treatment, but not estrogen. Likewise, ADH activity was approximately two-fold higher in progesterone-treated rat uteri compared with controls. This study demonstrated that ADH class I gene is progesterone-responsive in the uterus. This implies that progesterone might be involved with retinoic acid synthesis in the uterus, since ADH is the key enzyme for retinoic acid synthesis.

【 授权许可】

Unknown