Genetic diversity of citrus rootstocks based on RAPD marker analysis

Ciência Rural

Bastianel, Marinês³  Dornelles, Ana Lúcia Cunha²  Schäfer, Gilmar¹ 

[1] Universidade Federal do Rio Grande do Sul;Universidade Federal do Rio Grande do Sul, Porto Alegre;Universidade Estadual de Campinas

关键词: citrus;    Poncirus trifoliata;    Citrus limonia;   

DOI  :  10.1590/S0103-84782004000500017

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

This research intended to characterize the genetic diversity of citrus rootstocks from the Experimental Agronomic Station of the Rio Grande do Sul Federal University (EEA/UFRGS) and rootstocks collected in nurseries of the Cai River Valley - Rio Grande do Sul - Brazil. The method used was the Random Amplification of Polymorphic DNA (RAPD) markers. Leaves of nine citrus rootstocks from EEA/UFRGS and ten accesses of trifoliate orange (Poncirus trifoliata) from different nurseries were collected. With the use of nine random primers it was possible to separate the rootstocks in two main groups: one formed by ‘Rangpur’ lime (Citrus limonia) and the other by trifoliate orange and its hybrids of low genetic similarity. RAPD molecular marker was very efficient for the citrus rootstock characterization and for the trifoliate orange separation from its hybrids. The technique might be used to characterize cultivars, analysis of genetic variability among progenitors in improvement breeding programs of rootstock , and identify zygotic seedlings of trifoliate orange in commercial nurseries.

【 授权许可】

CC BY-NC