Apoptosis of Murine Hepatocytes Induced by High Doses of Galactosamine

Journal of Veterinary Medical Science

Seiji KAWAMURA²  Takeshi MIKAMI¹  Shigeki TSUTSUI²  Kunio DOI²  Ken MAEDA¹  Makio TAKEDA²  Shin-ichi ITAGAKI²  Kensuke HIRASAWA² 

[1] Departments of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo;Departments of Biomedical Science, Faculty of Agriculture, The University of Tokyo

关键词: apoptosis;    galactosamine;    hepatotoxicity;    liver;    mouse;   

DOI  :  10.1292/jvms.59.785

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(16)Apoptosis induced by high doses of Galactosamine (GalN) was investigated in mice hepatocytes in vivo. In mice intraperitoneally (ip) treated with GalN 3 g/kg, the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive cells were first observed at 6 hr postadministration (PA). Both acidophilic bodies in hematoxylin and eosin (HE)-stained sections and TUNEL-positive cells were markedly found at 24 hr PA. At 48 hr PA, cellular degeneration and necrosis of hepatocytes were prominently observed, and TUNEL-positive cells were scarcely found. In the mice ip treated with GalN 1.5 g/kg, the lesion was milder than that in those treated with GalN 3 g/kg. Acidophilic bodies and TUNEL-positive cells were scarcely found at 24 hr PA, whereas they were markedly seen at 48 hr PA. In addition, a ladder-like DNA fragmentation pattern by agarose gel electrophoresis was observed most remarkably at 24 hr PA with GalN 3 g/kg and at 48 hr PA with GalN 1.5 g/kg, and less distinctly at 48 hr PA with GalN 3 g/kg. On the other hand, sGOT and sGPT activities increased prominently at 48 hr PA with GalN 3 g/kg. These results suggest that the cell death induced by high dose of GalN may be caused by apoptosis, and subsequently by necrosis in vivo.

【 授权许可】

Unknown