Acute depletion of plasma membrane phospholipids—dissecting the roles of PtdIns(4)P and PtdIns(4,5)P2

Journal of Chemical Biology

Banafshé Larijani¹  Natali Fili²  Nirmal Jethwa¹ 

[1] Cell Biophysics Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln’s Inn Fields, London, WC2A 3LY UK;Institut für Zelluläre Physiologie, Physiologisches Institut, Ludwig Maximilians Universität München, 80336 Munich, Germany

关键词: Plasma membrane;    Phospholipids;    PI4P;    PtdIns(4;    5)P2;   

DOI  :  10.1007/s12154-012-0080-3

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

The study of signalling pathways and metabolic networks has long been assisted by tools which manipulate proteins: ectopic gene expression; gene knockouts or specific mutations; knockdown of expression levels by RNAi; or reductions in activity by chemical inhibitors. However, the relationship between a protein and its metabolite products (and the eventual biological effect) is complex. Biological membranes are typically composed of phospholipids which are synthesised, interconverted and recycled by a network of lipid metabolic enzymes [13]. These enzymes display a relative promiscuity for their substrates—both in terms of the headgroup (for example, MTM1 can dephosphorylate the 3-phosphate of PtdIns(3)P and PtdIns(3,5)P2) and the exact molecular species (the length, degree of saturation and combination of the fatty acyl chains). As a result, the potential pool of phospholipid species is incredibly diverse.

【 授权许可】

Unknown