Hepatic Carnitine Palmitoyltransferase Activity in Cattle

Journal of Veterinary Medical Science

Mamoru YAMAGUCHI²  Hisashi MIZUTANI¹  Toshinori SAKO¹  Hidekazu KOYAMA¹  Naoyuki TAKEMURA¹  Shigekatsu MOTOYOSHI¹ 

[1] Department of Veterinary Internal Medicine, Nippon Veterinary and Animal Science University;Muscle Biology Laboratory, Department of Veterinary Bioscience, Ohio State University

关键词: carnitine palmitoyltransferase;    cattle;    fluorometric assay;   

DOI  :  10.1292/jvms.59.1067

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(2)A fluorometric assay for the determination of hepatic carnitine palmitoyltransferase (CPT) activity was slightly modified for use with cattle samples. With this assay, the Km value was 0.56 ± 0.10 mM with respect to L-carnitine (mean ± SD, n=4) and was 9.6 ± 2.2 μM (n=3) with respect to palmitoyl-CoA. The average hepatic CPT activity was 33.6 ± 2.0 μmol CoASH/min/g protein in 38 healthy cattle and was similar in both sexes and among breeds. Hepatic CPT activity showed no correlation with serum phospholipid, free fatty acid, triglyceride or total cholesterol concentrations.

【 授权许可】

Unknown