Journal of Veterinary Medical Science | |
Hepatic Carnitine Palmitoyltransferase Activity in Cattle | |
Mamoru YAMAGUCHI2  Hisashi MIZUTANI1  Toshinori SAKO1  Hidekazu KOYAMA1  Naoyuki TAKEMURA1  Shigekatsu MOTOYOSHI1  | |
[1] Department of Veterinary Internal Medicine, Nippon Veterinary and Animal Science University;Muscle Biology Laboratory, Department of Veterinary Bioscience, Ohio State University | |
关键词: carnitine palmitoyltransferase; cattle; fluorometric assay; | |
DOI : 10.1292/jvms.59.1067 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(15)Cited-By(2)A fluorometric assay for the determination of hepatic carnitine palmitoyltransferase (CPT) activity was slightly modified for use with cattle samples. With this assay, the Km value was 0.56 ± 0.10 mM with respect to L-carnitine (mean ± SD, n=4) and was 9.6 ± 2.2 μM (n=3) with respect to palmitoyl-CoA. The average hepatic CPT activity was 33.6 ± 2.0 μmol CoASH/min/g protein in 38 healthy cattle and was similar in both sexes and among breeds. Hepatic CPT activity showed no correlation with serum phospholipid, free fatty acid, triglyceride or total cholesterol concentrations.
【 授权许可】
Unknown
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