Protective Effect of Clostridium septicum Alpha-Toxoid Vaccine against Challenge with Spores in Guinea Pigs

Journal of Veterinary Medical Science

Eiji OISHI¹  Toshiaki OHGITANI¹  Osamu SASAKI¹  Katsuhiko AMIMOTO¹  Sotoyuki OTA¹  Shigeji KATAYAMA¹  Makoto ISOGAI¹ 

[1] Division of Veterinary Microbiology, Kyoto Biken Laboratories

关键词: alpha-toxin;    Clostridium septicum;    toxoid;   

DOI  :  10.1292/jvms.64.67

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(6)The protective effect of an alpha-toxoid vaccine of Clostridium septicum purified alpha-toxin was investigated in guinea pigs. Purified alpha-toxin was treated with formalin to make toxoid, and alpha-toxoid vaccine was prepared by mixing alpha-toxoid (4 to 64 μg/dose) with an aluminum phosphate gel as adjuvant. Guinea pigs were immunized twice with different doses of alpha-toxoid vaccine, and challenged with spores of C. septicum. The guinea pigs surviving after challenge had been immunized with 8 μg/dose or more of alpha-toxoid. All these animals produced titers of 20 units or higher of antitoxin at the challenge. The results suggest that C. septicum alpha-toxin plays an important role in protection against challenge with spores in guinea pigs.

【 授权许可】

Unknown