Rapid Identification of Echinococcus granulosus and E. canadensis Using High-Resolution Melting (HRM) Analysis by Focusing on a Single Nucleotide Polymorphism

Japanese Journal of Infectious Diseases

Mohammadhasan Tajaddini³  Ahmad Hosseini Safa⁶  Nader Pestehchian¹  Mohammad Rostami-Nejad²  Majid Fasihi Harandi⁵  Mehdi Mohtashami-Pour⁴ 

[1] Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences;Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences;Physiology Research Center, Isfahan University of Medical Sciences;Department of Medical Parasitology and Mycology, Tabriz University of Medical Sciences;Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences;Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences

关键词: Echinococcus granulosus;    cox1 gene;    genotype;    high-resolution melting analysis;    Iran;   

DOI  :  10.7883/yoken.JJID.2015.161

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(37)Cited-By(1)High-resolution melting (HRM) is a reliable and sensitive scanning method to detect variation in DNA sequences. We used this method to better understand the epidemiology and transmission of Echinococcus granulosus. We tested the use of HRM to discriminate the genotypes of E. granulosus and E. canadensis. One hundred forty-one hydatid cysts were collected from slaughtered animals in different parts of Isfahan-Iran in 2013. After DNA extraction, the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was amplified using PCR coupled with the HRM curve. The result of HRM analysis using partial the sequences of cox1 gene revealed that 93, 35, and 2 isolates were identified as G1, G3, and G6 genotypes, respectively. A single nucleotide polymorphism (SNP) was found in locus 9867 of the cox1 gene. This is a critical locus for the differentiation between the G6 and G7 genotypes. In the phylogenic tree, the sample with a SNP was located between the G6 and G7 genotypes, which suggest that this isolate has a G6/G7 genotype. The HRM analysis developed in the present study provides a powerful technique for molecular and epidemiological studies on echinococcosis in humans and animals.

【 授权许可】

Unknown